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Why did Tye's eyes change from brown to blue? Was it due to the Reach's testing? I checked the episodes to see if it was a mistake, but before he was captured he had brown eyes and after he had blue in all of his appearances.
I never noticed. You'd have to double-check with Brandon Vietti to see if this was intentional or an accident. If it was the former, the reason was his. If it was the latter, then we could come up with an in-universe justification for it - like the one you suggest above.